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This video provides a step-by-step guide to determining nitrate and nitrite concentrations in a water sample, using a nitrate testing kit. It describes what chemical reactions are taking place throughout the testing process. The resource includes a video and a written transcript, and is supported by the Nitrate Protocol in the GLOBE Teacher's Guide. This is one of seven videos on hydrology in the 24-part instructional video series describing scientific protocols used by GLOBE (Global Learning and Observation to Benefit the Environment) a worldwide, hands-on, K-12 school-based science education program.